Part:BBa_K150003:Experience

To characterize the ability of this protein generator to mediate resistance against chloramphenicol we used E. coli Top10 transformed with the part BBa_K150003 and measured their ability to grow in LB medium containing different amounts of chloramphenicol (see figure 2). As control we used untransformed E. coli Top10 (see figure 1). To exclude the possibility that high concentrations of chloramphenicol led to the death of the bacteria only through the solvent ethanol we also measured the same ethanol concentrations as mock treated (see figure 3).
It was shown that this part leads to acceptable resistance up to 100 µg/ml chloramphenicol, even in 500 µg/ml unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.

Figure 1: Growth of untransformed E. coli Top 10 in LB medium containing the indicated amounts of chloramphenicol. Note that even 5 µg/ml chloramphenicol lead to hindert cell growth and cell death.

Figure 2: Growth of E. coli Top 10 transformed with BBa_K150003 in the indicated amounts of chloramphenicol. Note that this part leads to acceptable resistance up to 100 µg/ml. Even in 500 µg/ml chloramphenicol unmistakably cell growth can be detected.

Figure 3: Growth curve of mock treated E. coli Top 10 to examinate the effect of the solvent on the dying cells in figure 2. It can be seen that the effect of the solvent ethanol can be neglected.

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